Split GFP quantification provides direct quantification of protein expression using a split green fluorescent protein system. We use this technology for expression validation and qualitative yield estimation.

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How It Works

The split GFP system divides the green fluorescent protein into two non-fluorescent fragments. When these fragments are brought together through protein expression, they reconstitute to form a functional, fluorescent GFP. The fluorescence intensity correlates with the amount of expressed protein.

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Applications

Expression Validation

Confirm successful protein expression and validate expression systems.

Yield Estimation

Qualitative assessment of protein expression yields across different conditions.

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Integration

Split GFP quantification works alongside our other technologies to provide comprehensive protein expression analysis, complementing cell-free expression systems and other analytical methods for complete protein characterization workflows.