1

Experiment Configuration

The process begins when you submit your project details through the Foundry Portal. Our intuitive configurator allows you to specify:

  • Type of experiment: Choose from binding screening, affinity characterization, epitope binning, and more
  • Protein variants: Upload sequences or select from our library
  • Target molecules: Define the antigens or ligands of interest
  • Custom requirements: Set specific concentrations, controls, or replicates

After submission, our team reviews the feasibility of your project, creates a detailed project plan, and communicates any necessary adjustments or recommendations.

2

Build Phase

During the build phase, we prepare all necessary materials and reagents for your experiments. This includes:

DNA Synthesis and Optimization

  • Synthesis of DNA sequences encoding your protein variants
  • Codon optimization for efficient expression
  • Quality verification of synthesized constructs

Protein Expression

  • Production of protein variants using our cell-free expression system
  • High-quality, consistent protein yields
  • Verification of expression levels and quality

Target Preparation

  • Sourcing or production of target molecules
  • Access to our pre-curated antigen library or custom target preparation

Buffer and Reagent Preparation

  • Tailored to your experimental conditions
  • Optimized for stability and performance
3

Data Generation (Protein Testing)

This is where we test your proteins and targets using the experimental workflows you’ve selected. For binding experiments, we use bio-layer interferometry (BLI), a label-free technology that provides real-time binding kinetics.

Experiment Execution

  • Immobilization or presentation of proteins on biosensor tips
  • Introduction of target analytes in solution
  • Real-time monitoring of binding interactions

Technical Precision

  • Single or multiple technical replicates based on your requirements
  • Consistent experimental conditions across all samples

Controls

  • Inclusion of negative controls to assess non-specific binding
  • Positive controls to validate experimental setup

Quality Assurance

  • Continuous monitoring of experimental parameters
  • Flagging of unexpected results for review
4

Analysis, Validation, And Reporting

After data acquisition, we perform rigorous analysis and validation to ensure the accuracy and reliability of your results.

Data Processing

  • Fitting of raw sensorgrams to appropriate kinetic models
  • Extraction of key parameters (k_on, k_off, K_D values)
  • Statistical analysis of replicate data

Validation

  • Cross-checking results against controls
  • Verification of data consistency across replicates
  • Identification of any outliers or anomalies

Comprehensive Reporting

  • Detailed report including:
    • Raw data and processed results
    • Kinetic parameters and binding affinities
    • Experimental conditions and notes
    • Interpretation and recommendations

Foundry Portal Access

  • View and download raw sensorgrams
  • Explore fitted kinetic models
  • Filter and categorize results
  • Track project status in real-time
  • Access archived results anytime

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Frequently Asked Questions