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SPR measures biomolecular interactions in real time without labels. Use it to quantify kon, koff, and KD across a broad affinity range.

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What it measures

  • Real-time association and dissociation to quantify kon, koff, and KD
  • Label-free readout on sensor chips
SPR sensorgram showing association and dissociation across a target concentration series

Illustrative SPR sensorgram, simulated for a KD of 4 nM, across a target concentration series. Real-time association and dissociation yield kon, koff, and KD.

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When to use

  • You need precise kinetics beyond endpoint binding
  • Benchmarking or mechanism studies

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How we use SPR at Adaptyv

We use the Carterra LSA XT for high-throughput SPR measurements. Our typical workflow involves:
  • Protein expression: User-designed proteins are expressed with a linker and Twin-Strep-Tag
  • Surface capture: Tagged proteins are immobilized on the sensor chip surface
  • Kinetic analysis: Real-time binding measurements to determine kon, koff, and KD values
This approach allows us to efficiently characterize binding interactions for designed proteins across multiple conditions.