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What you get

  • Activity call: Active / low / not detected
  • Relative rate: Normalized activity per variant for fair comparison
  • Assay conditions: Substrate, buffer, temperature, and detection method

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Assay formats

  • Endpoint assays: Single time‑point readouts for high throughput
  • Kinetic assays: Time‑course measurements for rate estimation

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When to use

  • Early triage of designed enzymes to find active scaffolds
  • Optimization campaigns to compare mutations and conditions

Endpoint assay

High‑throughput single time‑point readouts for large libraries.

Kinetic assay

Time‑course data to estimate rates and compare variants.
Ready to set up an enzyme activity assay? Visit start.adaptyvbio.com/enzyme-activity.

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FAQs

Endpoint assays maximize throughput on large libraries. Kinetic assays give you a time course to estimate rates. Screen broadly with endpoint reads, then characterize the variants you care about with kinetic assays.
Often, yes. If your reaction is not already covered, contact us with the target reaction and a detection idea, and we will scope and quote the assay development.